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- Article name
- Biochemical and biophysical approaches to the analysis of factors affecting water quality. Research of enzymatic activity
- Authors
- OSTROUMOV S. A., , ar55@yandex.ru, M. V. Lomonosov Moscow State University, Moscow, Russia
KOTELEVTSEV S. V., , , M. V. Lomonosov Moscow State University, Moscow, Russia
SADCHIKOV A. P., , , International Biotechnology Center Moscow State University named after M. V. Lomonosov, Moscow, Russia
ORLOV S. N., , , Moscow State University named after M. V. Lomonosov, Moscow, Russia
- Keywords
- enzymatic activity / proteolitic activity / exoenzymes / biochemical and biophysical methods / freshwater ecosystems / phytoplankton / bacterioplankton / water filtration / water quality
- Year
- 2017 Issue 4 Pages 44 - 48
- Code EDN
- Code DOI
- Abstract
- In this paper, new methods to discover and characterize activity of exoenzymes in surface ecosystem water were developed. Enzyme activity was exemplified by proteolitic activity. The new methodology uses measurement of polarization of fluorescence of proteins labeled with fluoresceinisothiocyanate (FITC). This methodology allows measurement of proteolitic activity of exoenzymes in aquatic environment. Using this approach, enzymatic (proteolitic) activity was discovered in natural samples of water (with the natural community of plankton) from a surface freshwater ecosystem in the central region of the European part of Russia. Proteolitic activity was discovered in the water sample before and after removal of phytoplankton and bacterioplankton by water filtration. This provides evidence for making conclusion on the presence of exoenzymes in water medium.
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